The tissue types they tested that contained the stem-cell-derived cells included the brain, heart, kidney, liver, and gastrointestinal tract. They also used gene sequencing and other tests to confirm the presence of stem-cell-derived tissue across different organs. The investigators used the green fluorescent protein label to determine which tissues contained cells derived from the injected stem cells. The embryos were implanted into female macaques, resulting in 12 pregnancies and six live births.Īnalysis confirmed that one monkey that was born alive and one fetus that was miscarried were substantially chimeric, containing cells that grew out of the stem cells throughout their bodies. Ultimately, they selected a particular subset of stem cells to inject into early monkey morula embryos (embryos that are 4-5 days old). The stem cells were also labeled with green fluorescent protein so the researchers would be able to determine which tissues had grown out of the stem cells in any animals that developed and survived. They performed a number of different tests on the cells to confirm that they were pluripotent - having the ability to differentiate into all of the cell types needed to create a live animal. They then placed the cell lines in culture to give them enhanced ability to differentiate into different cell types. The investigators first established nine stem cell lines using cells removed from 7-day-old blastocyst embryos.

The monkeys used in the study were cynomolgus monkeys, also known as crab-eating or long-tailed macaques, a primate common in biomedical research.

Specifically, this work could help us to generate more precise monkey models for studying neurological diseases as well as for other biomedicine studies.' 'This research not only has implications for understanding naive pluripotency in other primates, including humans, but it also has relevant practical implications for genetic engineering and species conservation. * I looked around at various fish enthusiast forums, and there seems to be some support for GE silicon 1 being aquarium safe even though it doesn't claim to be, I probably would have used that (even though i had nightmares of killing all my sea monkeys trying to improve their quality of life) but my hardware store only sold this in large containers, and since they had the dap 'aquarium safe' silicone in a small tube i went with it.'This is a long-sought goal in the field,' says senior author Zhen Liu of the Chinese Academy of Sciences (CAS). I found the sand and gravel much easier to work with while wet. At this time i also drained my glass and set it on a towel to cool and dry. *īefore doing anything else, I cut a square of parchment paper in the dimension of my tank (i did this by inverting the tank and tracing around it then cutting inside the line, but you could simply measure too). I also invested in a tube of silicone 'adhesive sealant' that stated its 'aquarium safe'. Make sure you add your glass before bringing the water to boil, and boil gently. I could find no difference noted on the packaging for certain things (like gravel, sand, and glass), but decided to err on the side of caution, and boiled all these things after rinsing them.

i decided to visit the dollar store instead. After seeing the exorbitant prices on aquarium stuff.

Live seamonkey